000014152 001__ 14152
000014152 005__ 20161115100143.0
000014152 04107 $$aeng
000014152 046__ $$k2016-08-21
000014152 100__ $$aHuo, Bo
000014152 24500 $$aMechanical status within cell monolayer decides the fusion of osteoclast precursors

000014152 24630 $$n24.$$p24th International Congress of Theoretical and Applied Mechanics - Book of Papers
000014152 260__ $$bInternational Union of Theoretical and Applied Mechanics, 2016
000014152 506__ $$arestricted
000014152 520__ $$2eng$$aIt is always a confusing issue why bone resorption occurs at the site of microdamage within bone, where osteoclast precursors aggregate and finally fuse into osteoclasts. We hypothesize that the mechanical status within cell monolayer correlating with its morphology decides the cell fusion. In this study, the micro-contact printing technology was adopted to build multicellular monolayer with circular pattern. Traction force observations showed that the cell-substrate interaction reached its maximum value at the outer edge, interestingly, where the expression of force-transferring proteins, F-actin (intracelluar tension), vinculin (cell-substrate interaction), !-catenin and E-cadherin (cell-cell connections), were also highest. Probably responding to this high-level in-plane shear stress, the cell fusion was lowest at the outer edge of circular patterns. These results indicate that the mechanical microenvironment within the cell monolayer plays an important role in the fusion of osteoclast precursors and provide insight into the molecular mechanism.

000014152 540__ $$aText je chráněný podle autorského zákona č. 121/2000 Sb.
000014152 653__ $$a

000014152 7112_ $$a24th International Congress of Theoretical and Applied Mechanics$$cMontreal (CA)$$d2016-08-21 / 2016-08-26$$gICTAM2016
000014152 720__ $$aHuo, Bo
000014152 8560_ $$ffischerc@itam.cas.cz
000014152 8564_ $$s55914$$uhttps://invenio.itam.cas.cz/record/14152/files/PO.SM01-1.08.199.pdf$$yOriginal version of the author's contribution as presented on CD,  page 1721, code PO.SM01-1.08.199
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000014152 962__ $$r13812
000014152 980__ $$aPAPER