000014810 001__ 14810
000014810 005__ 20161115100202.0
000014810 04107 $$aeng
000014810 046__ $$k2016-08-21
000014810 100__ $$aReichert, Peter
000014810 24500 $$aAcoustophoretic Handling of C. elegans in Microfluidic Channels

000014810 24630 $$n24.$$p24th International Congress of Theoretical and Applied Mechanics - Book of Papers
000014810 260__ $$bInternational Union of Theoretical and Applied Mechanics, 2016
000014810 506__ $$arestricted
000014810 520__ $$2eng$$aCaenorhabditis elegans (C. elegans) is a model organism for research in the field of molecular and developmental biology. Microfluidic chips have improved the handling capabilities and have made high-resolution imaging and fluorescent scanning of C. elegans possible by immobilization with special geometries, cooling or gasses [1]. Bulk acoustic wave (BAW) acoustophoresis is proposed to be a new contactless handling tool for un-anesthetized C. elegans with ultrasound. Acoustophoresis has been used to position or rotate particles in a fluid [2]. Here we show the abilities of this method for the contactless and harmless immobilization of C. elegans. The C. elegans are injected into a silicon microchanel and immobilized by focussing them in the pressure nodal lines of a standing acoustic wave. Switching between two fluid resonance modes is used to sort C.elegans in two separate outlets. Moreover, a 3D numerical simulation shows the physical princples of C. elegans manipulation.

000014810 540__ $$aText je chráněný podle autorského zákona č. 121/2000 Sb.
000014810 653__ $$a

000014810 7112_ $$a24th International Congress of Theoretical and Applied Mechanics$$cMontreal (CA)$$d2016-08-21 / 2016-08-26$$gICTAM2016
000014810 720__ $$aReichert, Peter
000014810 8560_ $$ffischerc@itam.cas.cz
000014810 8564_ $$s100628$$uhttps://invenio.itam.cas.cz/record/14810/files/TS.FS01-2.04.pdf$$yOriginal version of the author's contribution as presented on CD,  page 3055, code TS.FS01-2.04
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000014810 962__ $$r13812
000014810 980__ $$aPAPER