000014847 001__ 14847
000014847 005__ 20161115100204.0
000014847 04107 $$aeng
000014847 046__ $$k2016-08-21
000014847 100__ $$aWu, Shangquan
000014847 24500 $$aCell viability and rapid screening anti-cancer drug based on nanomechanical fluctuation

000014847 24630 $$n24.$$p24th International Congress of Theoretical and Applied Mechanics - Book of Papers
000014847 260__ $$bInternational Union of Theoretical and Applied Mechanics, 2016
000014847 506__ $$arestricted
000014847 520__ $$2eng$$aWe present an approach of utilizing a nanomechanical fluctuation based highly sensitive microcantilever sensor, which is capable of characterizing the viability of cells and quantitatively screening (within tens of minutes) their responses to a drug with the obvious advantages of real-time and in-situ assay. The microcantilever sensor operated influctuation mode was used in evaluating the paclitaxel effectiveness on breast cancer cell line MCF-7. This study demonstrated that the nanomechanical fluctuations of the microcantilever sensor are sensitive enough to detect the dynamic variation in cellular force which is provided by the cytoskeleton, using cell metabolism as its energy source, and the dynamic instability of microtubules plays an important role in the generation of the force. We propose that cell viability consists of two parts: biological viability and mechanical viability. This new method provides a new concept and strategy for the evaluation of cell viability and the screening of anti-cancer drugs.

000014847 540__ $$aText je chráněný podle autorského zákona č. 121/2000 Sb.
000014847 653__ $$a

000014847 7112_ $$a24th International Congress of Theoretical and Applied Mechanics$$cMontreal (CA)$$d2016-08-21 / 2016-08-26$$gICTAM2016
000014847 720__ $$aWu, Shangquan
000014847 8560_ $$ffischerc@itam.cas.cz
000014847 8564_ $$s139546$$uhttps://invenio.itam.cas.cz/record/14847/files/TS.FS03-3.05.pdf$$yOriginal version of the author's contribution as presented on CD,  page 3158, code TS.FS03-3.05
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000014847 962__ $$r13812
000014847 980__ $$aPAPER