000015042 001__ 15042
000015042 005__ 20161115100209.0
000015042 04107 $$aeng
000015042 046__ $$k2016-08-21
000015042 100__ $$aXu, Feng
000015042 24500 $$aCell alignment fabrication using stretchable hydrogels with programmable strain gradients

000015042 24630 $$n24.$$p24th International Congress of Theoretical and Applied Mechanics - Book of Papers
000015042 260__ $$bInternational Union of Theoretical and Applied Mechanics, 2016
000015042 506__ $$arestricted
000015042 520__ $$2eng$$aThe mechanical gradients (e. g., stiffness and stress/strain gradients) of extracellular matrix play an important role in guiding cellular alignment, especially in tendons and muscles. Though various methods have been developed to engineer graded mechanical environment to study its effect on cellular behaviours, most of them failed to distinguish stiffness effect from stress/strain effect during mechanical loading. Here, we construct a mechanical environment with strain gradients by using a hydro gel of a linear elastic property. We demonstrate that the pattern of cellular alignment can be rather precisely tailored by substrate strains. The experiment is in consistency with a theoretical prediction when assuming that focal adhesions would drive cell to reorient to the directions where they are most stable. This work not only provides important insights into the cellular response to local mechanical microenvironment, but can also be utilized to engineer patterned cellular alignment that can be critical in tissue remodelling applications.

000015042 540__ $$aText je chráněný podle autorského zákona č. 121/2000 Sb.
000015042 653__ $$a

000015042 7112_ $$a24th International Congress of Theoretical and Applied Mechanics$$cMontreal (CA)$$d2016-08-21 / 2016-08-26$$gICTAM2016
000015042 720__ $$aXu, Feng
000015042 8560_ $$ffischerc@itam.cas.cz
000015042 8564_ $$s158350$$uhttps://invenio.itam.cas.cz/record/15042/files/TS.MS05-3.05.pdf$$yOriginal version of the author's contribution as presented on CD,  page 272, code TS.MS05-3.05
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000015042 962__ $$r13812
000015042 980__ $$aPAPER