000015099 001__ 15099
000015099 005__ 20161115100211.0
000015099 04107 $$aeng
000015099 046__ $$k2016-08-21
000015099 100__ $$aCrone, Wendy
000015099 24500 $$aInfluence of Microscale Patterning and Substrate Stiffness on Cardiomyocyte Maturation

000015099 24630 $$n24.$$p24th International Congress of Theoretical and Applied Mechanics - Book of Papers
000015099 260__ $$bInternational Union of Theoretical and Applied Mechanics, 2016
000015099 506__ $$arestricted
000015099 520__ $$2eng$$aRecent advancements in methods of deriving immature human cardiomyocytes from pluripotent stem cells now facilitates the potential of these cells to develop into mature, fillly-fimctional adult cardiomyocytes - the next step towards producing usefill drug screening models. Differentiation protocols are now able to achieve both high yield and purity of stem cell derived cardiomyocytes. However, biochemical signaling alone is insufficient to produce well-aligned, mature cardiomyocytes that recapitulate adult cell functionality. Our work using hESCs explores the use of micropattemed substrates and substrate stiffness to improve the cytoskeletal organization of the immature cardiomyocytes to improve maturation. We show that pattern widths ranging from 30-230ij promote significant increases in nuclear alignment and enhanced myofilament structure due to the feature geometries. Results for immature CMs cultured on patterned PDMS substrates are presented, in comparison to our prior work on glass, showing the portability of this technique between substrates.

000015099 540__ $$aText je chráněný podle autorského zákona č. 121/2000 Sb.
000015099 653__ $$a

000015099 7112_ $$a24th International Congress of Theoretical and Applied Mechanics$$cMontreal (CA)$$d2016-08-21 / 2016-08-26$$gICTAM2016
000015099 720__ $$aCrone, Wendy
000015099 8560_ $$ffischerc@itam.cas.cz
000015099 8564_ $$s156819$$uhttps://invenio.itam.cas.cz/record/15099/files/TS.SM01-3.04.pdf$$yOriginal version of the author's contribution as presented on CD,  page 1684, code TS.SM01-3.04
.
000015099 962__ $$r13812
000015099 980__ $$aPAPER