Damage of muscle cells under mechanical and oxidative stresses

Abstract eng:
Deep tissue injury due to prolonged excessive loadings can lead to clinical pressure ulcers. We assess the duration-dependent damage thresholds of muscle cells in compression and shear using myoblasts in-vitro, and study the effects of oxidative stress on such thresholds. We study the effects of oxidative stress on cytoskeletal polymerization and use AFM to study the corresponding changes in cell stiffness. We use femtosecond laser to evaluate how oxidative stress affects the pre-tension in the cytoskeletal fibers and the ability of cells to repair submicron-pores on their plasma membranes. Our findings suggest interesting implications of damage vulnerability under prolonged oxidative exposures, such as during chronic inflammation. Using a mouse model, we examine the damages incurred by a prolonged exposure to epidermal pressure and how a surfactant cell membrane stabilizer poloxamer-188 and intermittent vibration may alleviate these damages.

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