Cell-selective electroporation for novel single-cell applications

Abstract eng:
A robust method for single cell access to deliver genes and molecules into adhered cells is needed to enable novel capabilities for temporal biological studies and new approaches to in vitro cell analysis, cell cloning, and gene editing. To realize this goal, we developed a microfluidic nanofountain probe (N FP) technology for localized electroporation of individual cells via automated positioning of the probe in contact with the target cell. Our recent development has increased ease-of-use and throughput for single-cell transfection.

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