Ultrasonic Force Potential Wells for Trapping Arrays of Single Cells - One Cell Per Well (OCPW)

Abstract eng:
Single cell analysis enables the study of cellular activity and parameters at an individual, rather than population-average basis. This is made possible by retaining cells so that temporal data can be accessed. In contrast to previous capture approaches utilising mechanical traps, we present an ultrasonic method to capture and release spatially separated cells on demand, with potential for population screening and analysis. The transition from manipulation of particles in groups, which is routinely performed using ultrasound, to individuals requires the reduction of ultrasonic wavelength, but it also introduces a unique physical situation. This arises from the two key forces present: the acoustic radiation force and Bjerknes forces. The former collects particles at defined locations within the sound field, whereas the latter acts to coagulate particles. For collection of individual particles the former is beneficial, the later a hindrance; we show that over a defined range of parameters single particle and cell capture is possible.

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